Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. The site is secure. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. official website and that any information you provide is encrypted Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. (1954). Exp Cell Res. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. WebTry out PMC Labs and tell us what you think. Food Funct. Pure Appl. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. The term f/f0 is sometimes denoted as P, Perrin constant. (2013). International Journal of Pharmaceutics, 277, 99-104. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. 4K and M.W. FITC-dextran solution (600L) was added to the donor chamber. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Herwadkar A, Banga AK. 8600 Rockville Pike The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. ; Yevlampieva N.P. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The Viscosity of Dilute Solutions of Long-Chain Molecules. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Carbohydrate Polymers, 74, 372-378. dextran With FITC-dextran 20 K, cumulative We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Cell permeabilization using a non-thermal plasma - IOPscience Drug Discov Today Technol. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Nanoparticles in magnetic resonance imaging: from simple to dual what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. Journal of Colloid Science, 31, 308-328. Cookies policy. Fixable: Fixable via Lysine. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Journal of Applied Polymer Science, 13, 2031-2032. Hydrodynamic radii ( RH) were determined by dynamic light scattering. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Front Microbiol. dextran Critical parameters for gel permeation Journal Non-Newtonian Fluid Mech., 97, 295-301. The receptor chamber was filled with PBS. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. J Appl Toxicol. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). Keywords: To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. Walkenhorst, S.; Olivier, R. (2002). Walkenhorst, S.; Olivier, R. (2003). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Table 2 compares the properties of nanoparticles coated with one or other polymer. The mechanism of the dextran-induced red blood cell Aggregation. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Advanced Drug Delivery Reviews, 60, 1650-1662. Synthesis and characterization of blue dextrans Kasaai, Mohammad R. (2012). 7 8 Garcia de la Torre, J.; Carrasco, B. Rheology of dextran solutions. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Learn More. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. Solvent effects on polysaccharide conformation. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. Unauthorized use of these marks is strictly prohibited. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Manage cookies/Do not sell my data we use in the preference centre. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. Pharmaceut Sci Tech. Treatment of acne scars by fractional bipolar radiofrequency energy. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Principles of Size Exclusion Chromatography SEC (GFC Ortega, A.; Garca de la Torre, J. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Nat Biotechnol. Radius Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. (2008). The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Anal. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Radiofrequency ablation of malignant liver tumors. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Epub 2017 Jun 1. Beer, M.U. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Sung-Wook Choi. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Webdextran hydrodynamic radius. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. 2010;4(1):117. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. This conjecture found to be correct. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). ; Wood, P.J. In fact, short chains do contain minor branching points. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. (2007). Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Optical properties of dextran in solution and in films. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. 4D imaging reveals mechanisms of clay-carbon protection and Polysaccharide hydrogels for modified release formulations. 2011;5(2):95132. (1998). ; Bataille, B. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Transdermal drug delivery: overcoming the skins barrier function. Trop J Pharm Res. Ibrahim O, Munavalli GS, Dover JS. Biological skin substitutes for wound cover and closure. Soc., 21, 1, 78-86. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Pharm Res. Amersham Biosciences, AA Edition, www.amershambiosciences.com. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). ; van Nostrum, C.F. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Colloid and Polymer Science, 286, 1223-1231. J. Phys. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Increased epidermal laser fluence through simultaneous ultrasonic microporation. 2000;3(9):31826. 10K, and c M.W. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Microneedles and their applications. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. Hydrodynamic Not for use in diagnostic procedures. If there are particles in the sample, the incident laser light gets scattered in all directions. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system.
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